Hydrogen-Form Columns
For organic acids, fermentation products and ion-exclusion separations.
Benson Polymeric product portfolio
Benson Polymeric HPLC Columns
Benson Polymeric columns are polymer-based HPLC columns developed for demanding carbohydrate, sugar, fermentation and organic-acid separations. Select the appropriate ionic form, cross-link density and column dimension according to the analytes, separation mechanism and compendial method.
Choose by ionic form
Benson Polymeric columns by separation selectivity
Each ionic form provides different selectivity for sugars, sugar alcohols, fermentation products and organic acids.
Calcium-Form Columns
For routine carbohydrate, monosaccharide and disaccharide analysis.
Sodium-Form Columns
For selected carbohydrates, sugar alcohols and USP L58 methods.
Lead-Form Columns
For complex carbohydrate mixtures and USP L34 applications.
Potassium-Form Columns
For specialized carbohydrate selectivity and method development.
Silver-Form Columns
For specialized carbohydrate separations requiring silver-ion selectivity.
Hydrogen form · H+
Benson Polymeric Hydrogen-Form HPLC Columns
Benson Polymeric hydrogen-form HPLC columns are designed for ion-exclusion and size-influenced separations of organic acids, alcohols, fermentation products and selected carbohydrates. The hydrogen ionic form is particularly useful where weak organic acids must be separated from neutral compounds in food, beverage, biotechnology and pharmaceutical samples. Benson H-form columns are available across relevant BP series and include products associated with USP L17 applications. Their polymeric stationary phase provides chemical stability and reproducible selectivity when the method is operated within the recommended temperature, flow-rate and mobile-phase limits. Typical applications include monitoring lactic, acetic, citric, succinic and other organic acids, as well as ethanol and residual sugars in fermentation broths. For best performance, samples should be filtered and free from particulates or strongly retained contaminants. Dilute aqueous acid eluents are commonly used, subject to the validated method. Choose the series and cross-link density according to the required resolution, analysis time and sample matrix. Review the individual product table for available dimensions and part numbers, and use a matching guard column whenever complex samples may shorten analytical-column life.
View hydrogen-form columns and specifications →Calcium form · Ca2+
Benson Polymeric Calcium-Form HPLC Columns
Benson Polymeric calcium-form HPLC columns are widely suited to the analysis of common carbohydrates, including monosaccharides, disaccharides and selected oligosaccharides. Calcium-form polymeric columns provide characteristic ligand-exchange and size-related selectivity that is frequently applied to food, beverage, dairy, sweetener and fermentation samples. Depending on the Benson series and cross-link density, these columns can support routine sugar profiling as well as methods associated with USP L19. Water is often used as the mobile phase in established carbohydrate methods, while elevated column temperature may improve peak shape, mass transfer and resolution. The exact operating conditions must follow the validated method and the specifications of the selected column. Typical analytes may include glucose, fructose, sucrose, lactose, maltose and related sugars, although suitability should always be confirmed with the actual sample matrix. Calcium-form columns benefit from careful sample preparation, filtration and protection against salts, proteins, oils and insoluble matter. A compatible guard column can reduce contamination of the analytical bed. Compare BP-100 and BP-800 calcium-form options by dimension, part number, cross-link density and intended application before selecting a column for method transfer or new method development.
View calcium-form columns and specifications →Sodium form · Na+
Benson Polymeric Sodium-Form HPLC Columns
Benson Polymeric sodium-form HPLC columns provide a useful alternative selectivity for carbohydrate and sugar-alcohol analysis. Sodium-form polymeric stationary phases can be applied when a calcium- or lead-form column does not provide the required separation pattern, or when a compendial procedure specifies a sodium-form phase. Benson sodium columns are available in selected BP series and include options associated with USP L58 applications. They may be considered for sugars, polyols and related polar compounds in food, beverage, pharmaceutical and industrial samples. The polymeric packing supports aqueous operation and is offered in different cross-link densities, allowing the chromatographer to balance permeability, exclusion behaviour and selectivity. Operating temperature can have a significant effect on viscosity, retention and peak efficiency, so conditions should be optimized only within the product limits and the validated method. Samples should be clarified and filtered, and highly contaminated matrices should be treated by suitable cleanup procedures. Use of a matching guard column is recommended where matrix components could foul the stationary phase. Review the sodium-form product page for available column dimensions, part numbers, series, USP designation and application notes before placing an order or transferring an existing method.
View sodium-form columns and specifications →Lead form · Pb2+
Benson Polymeric Lead-Form HPLC Columns
Benson Polymeric lead-form HPLC columns are designed for high-resolution separations of carbohydrates where lead-ion selectivity offers advantages for complex sugar mixtures. They are commonly considered for mixtures containing monosaccharides, disaccharides and oligosaccharides that may be difficult to resolve on other ionic forms. Benson lead-form columns are available in BP-100, BP-700 and BP-800 families with different polymer cross-link densities and include products associated with USP L34 applications. The choice between series should be based on the required selectivity, sample composition, operating pressure, temperature and validated method. Lead-form phases are particularly relevant to food, beverage, fermentation, sweetener and carbohydrate research laboratories. As with all ion-exchange polymeric columns, mobile-phase purity and sample cleanliness are important. Filter samples, avoid insoluble material and minimize substances that may irreversibly contaminate or alter the ionic form of the packing. A compatible guard column can protect the analytical column and help preserve performance. Consult the individual Benson lead-form page for catalog dimensions and part numbers. Technical review is recommended before replacing another brand or transferring a regulated method, because equivalent dimensions alone do not guarantee identical selectivity.
View lead-form columns and specifications →Potassium form · K+
Benson Polymeric Potassium-Form HPLC Columns
Benson Polymeric potassium-form HPLC columns provide specialized ionic selectivity for selected carbohydrate separations and method-development challenges. Potassium-form polymeric phases may be evaluated when sodium, calcium or lead forms do not deliver the required elution order or resolution for a particular group of sugars. Benson potassium columns are available in selected BP-100 and BP-800 configurations, allowing laboratories to compare cross-link density, column dimensions and application suitability. Their use may be relevant in food chemistry, fermentation monitoring, sweetener analysis and research involving structurally similar carbohydrates. Because the separation depends on a combination of ion interaction, ligand exchange, molecular size and temperature, method conditions should be optimized carefully and kept within the column specifications. High-purity aqueous mobile phases and well-prepared samples support stable operation. Samples containing proteins, oils, suspended solids or high levels of interfering salts may require pretreatment before injection. A corresponding guard column is advisable for routine analysis of complex matrices. The potassium-form product page lists the available catalog products and part numbers. For a new application or replacement method, provide the analyte list, existing conditions, detector and sample matrix to Akira Analytical Solutions so the most appropriate Benson configuration can be reviewed.
View potassium-form columns and specifications →Silver form · Ag+
Benson Polymeric Silver-Form HPLC Columns
Benson Polymeric silver-form HPLC columns are specialized polymeric columns intended for carbohydrate separations where silver-ion interactions provide distinctive selectivity. Silver-form phases can be useful for challenging mixtures in which closely related sugars are insufficiently resolved by more commonly used calcium, sodium or lead forms. Benson offers silver-form products in selected BP families, including BP-100 Ag and BP-200 Ag configurations, with differences in cross-link density and catalog dimensions. These columns may support research, food analysis, sweetener characterization and other applications requiring alternative carbohydrate selectivity. Because silver-form stationary phases are specialized, method conditions, sample composition and mobile-phase purity require careful control. Samples should be thoroughly filtered and should not introduce substances that could poison, precipitate with or alter the ionic form of the stationary phase. Avoid unvalidated additives and follow the recommended operating limits. A matched guard column is valuable for protecting the analytical bed from strongly retained matrix components. Before selecting a silver-form column, compare the target analytes, expected elution pattern, required resolution and existing method. The individual product page provides dimensions and part numbers, while technical support can assist with evaluating suitability for a specific separation or replacement requirement.
View silver-form columns and specifications →Choose by series
Benson BP column families
BP-100 Series
Six-percent cross-linked polymeric columns offered in multiple ionic forms for carbohydrate and organic-acid applications.
BP-200 Series
Four-percent cross-linked polymeric columns with selected ionic forms for carbohydrate separations.
BP-700 Series
Seven-percent cross-linked hydrogen- and lead-form columns for fermentation and carbohydrate analysis.
BP-800 Series
Eight-percent cross-linked columns in multiple ionic forms for robust analytical applications.
BP-OA Columns
Hydrogen-form polymeric columns developed for organic-acid and fermentation analysis.
Guard Columns
Matched guard formats for protecting Benson analytical columns from sample contamination.